万人炸金花怎么刷金币
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GST標簽蛋白純化預裝柱

時間:2018-02-01 14:24:57 瀏覽:

GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

  • High binding capacity.
  • Mild elution conditions preserving protein antigenicity and function.
  • Easy one-step purifications of Glutathione S-Transferase (GST) tagged proteins resulting in high purity.
  • GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as ?KTA design.

GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The resin, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ?KTA design or FPLC System.

PRODUCT PRODUCT NAME PRICE
28401745 GSTrap 4B, 5 x 1 ml 5 x 1 ml 337.00 USD
28401747 GSTrap 4B, 1 x 5 ml 1 x 5 ml 320.00 USD
28401748 GSTrap 4B, 5 x 5 ml 5 x 5 ml 1 240.00 USD
29048609 GSTrap 4B, 1 x 1 ml 1 x 1 ml 84.84 USD



Parameter GSTrap 4B, 1 x 1 ml
Chromatography technique GST-tagged protein purification 
Bed Dimensions 7 × 25 mm 
Bed Volume 1 ml
Flow Rate1 < 4 ml/min 
Storage 4 to 30°C, 20% Ethanol 
Pressure Max Over the Packed Bed During Operation 5 bar [0.5 MPa] (70 psi) 
Application GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. 
Bed Height 25 mm 
Column id 7 mm
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1 PreScission 蛋白酶 234 2017-11-28
2 GE 蛋白純化 133 2017-11-28
3 DUB-Detector? 82 2017-11-24
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